ythdf1 antibody  (Proteintech)

Journal: iScience

Article Title: N6-methyladenosine (m 6 A) modification of TXNIP in 3′UTR instigates abdominal aorta aneurysm in mice

doi: 10.1016/j.isci.2026.114630

Figure Lengend Snippet: TXNIP translation is dependent on the m 6 A reader YTHDF1 in hASMCs (A and B) Representative western blots and quantification of TXNIP expression in YTHDF1-silenced hASMCs following AngII (1 μM) treatment ( n = 3 samples/group). (C) RT-qPCR analysis of TXNIP mRNA in YTHDF1-silenced hASMCs ( n = 3 samples/group). (D) Representative western blot showing the successful immunoprecipitation of YTHDF1 protein in RIP samples using an anti-YTHDF1 antibody. Immunoglobulin G (IgG) served as the negative control ( n = 3 samples/group). (E) RT-qPCR analysis of TXNIP mRNA enrichment in RIP assays using an anti-YTHDF1 antibody ( n = 3 samples/group). Values are represented as mean ± SD; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 vs. sicontrol; ## p < 0.01 vs. siYTHDF1.

Article Snippet: anti-YTHDF1 , Proteintech , Cat#17479-1-AP.

Techniques: Western Blot, Expressing, Quantitative RT-PCR, Immunoprecipitation, Negative Control

Journal: Journal of Inflammation Research

Article Title: Machine Learning and Experimental Validation of m6A RNA Methylation Related Signatures for Risk Prediction, Diagnostic Biomarkers, and Immune Subtypes in Chronic Kidney Disease

doi: 10.2147/JIR.S553934

Figure Lengend Snippet: The results of in vitro experiment validation. ( A ) HE and Masson staining. ( B ) RT-PCR analysis of CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression levels (n=3). ( C ) CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression was detected via immunofluorescent. * P <0.05, ** P <0.01.

Article Snippet: The primary antibodies used in the study included: METTL3 (Proteintech, 15073-1-AP), CBLL1 (Proteintech, 21179-1-AP), ELAVL1 (Proteintech, 11910-1-AP), YTHDF1 (Proteintech, 17479-1-AP), RBM15B (Proteintech, 22249-1-AP).

Techniques: In Vitro, Biomarker Discovery, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Journal of Inflammation Research

Article Title: Machine Learning and Experimental Validation of m6A RNA Methylation Related Signatures for Risk Prediction, Diagnostic Biomarkers, and Immune Subtypes in Chronic Kidney Disease

doi: 10.2147/JIR.S553934

Figure Lengend Snippet: Analysis of immune cell infiltration. ( A ) Heat map of correlation between the expression level of m6A-related genes and immune cell infiltration. ( B ) Analysis of the difference in immune cell content in two m6A related subtypes. Box plot showed the difference of immune cell content in different expression groups of CBLL1 ( C ), ELAVL1 ( D ), YTHDF1 ( E ), RBM15B ( F ). ns, not significant; * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: The primary antibodies used in the study included: METTL3 (Proteintech, 15073-1-AP), CBLL1 (Proteintech, 21179-1-AP), ELAVL1 (Proteintech, 11910-1-AP), YTHDF1 (Proteintech, 17479-1-AP), RBM15B (Proteintech, 22249-1-AP).

Techniques: Expressing

Journal: Journal of Inflammation Research

Article Title: Machine Learning and Experimental Validation of m6A RNA Methylation Related Signatures for Risk Prediction, Diagnostic Biomarkers, and Immune Subtypes in Chronic Kidney Disease

doi: 10.2147/JIR.S553934

Figure Lengend Snippet: The results of in vitro experiment validation. ( A ) HE and Masson staining. ( B ) RT-PCR analysis of CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression levels (n=3). ( C ) CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression was detected via immunofluorescent. * P <0.05, ** P <0.01.

Article Snippet: The primary antibodies used in the study included: METTL3 (Proteintech, 15073-1-AP), CBLL1 (Proteintech, 21179-1-AP), ELAVL1 (Proteintech, 11910-1-AP), YTHDF1 (Proteintech, 17479-1-AP), RBM15B (Proteintech, 22249-1-AP).

Techniques: In Vitro, Biomarker Discovery, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Journal of Inflammation Research

Article Title: Machine Learning and Experimental Validation of m6A RNA Methylation Related Signatures for Risk Prediction, Diagnostic Biomarkers, and Immune Subtypes in Chronic Kidney Disease

doi: 10.2147/JIR.S553934

Figure Lengend Snippet: Analysis of immune cell infiltration. ( A ) Heat map of correlation between the expression level of m6A-related genes and immune cell infiltration. ( B ) Analysis of the difference in immune cell content in two m6A related subtypes. Box plot showed the difference of immune cell content in different expression groups of CBLL1 ( C ), ELAVL1 ( D ), YTHDF1 ( E ), RBM15B ( F ). ns, not significant; * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: The primary antibodies used in the study included: METTL3 (Proteintech, 15073-1-AP), CBLL1 (Proteintech, 21179-1-AP), ELAVL1 (Proteintech, 11910-1-AP), YTHDF1 (Proteintech, 17479-1-AP), RBM15B (Proteintech, 22249-1-AP).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, NMDAR2B, and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: Membranes were blocked with 5% non-fat milk for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies against METTL3 (1:1000; A19079; Abclonal), NMDAR2B (1:1000; AF6426; Affinity), YTHDF1 (1:1000; 17479-1-AP; Proteintech), and β-actin (1:100,000; 81115-1-RR; Proteintech).

Techniques: In Vivo, In Vitro, Methylation, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Modification

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: The roles of METTL3 and NMDAR2B in the anti-inflammatory effects of magnetic stimulation in vitro . (A) Effects of inhibition and overexpression of METTL3 on the protein bands of METTL3, NMDAR2B, and YTHDF1. (B–D) Western blot analyses of METTL3, YTHDF1, and NMDAR2B protein expression. (E) Effects of inhibition and overexpression of NMDAR2B on the protein bands of METTL3, NMDAR2B, and YTHDF1 (F–H) Western blot analyses of METTL3, YTHDF1, and NMDAR2B protein expression. (I–R) ELISA was used to detect the methylation modification level of m6A, NLRP3, IL-6, TNF-α, and IL-10. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: Membranes were blocked with 5% non-fat milk for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies against METTL3 (1:1000; A19079; Abclonal), NMDAR2B (1:1000; AF6426; Affinity), YTHDF1 (1:1000; 17479-1-AP; Proteintech), and β-actin (1:100,000; 81115-1-RR; Proteintech).

Techniques: In Vitro, Inhibition, Over Expression, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Methylation, Modification

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: Membranes were blocked with 5% non-fat milk for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies against METTL3 (1:1000; A19079; Abclonal), NMDAR2B (1:1000; AF6426; Affinity), YTHDF1 (1:1000; 17479-1-AP; Proteintech), and β-actin (1:100,000; 81115-1-RR; Proteintech).

Techniques: In Vitro, Immunofluorescence, Staining